rabbit polyclonal anti arf6  (Proteintech)

Journal: PLoS ONE

Article Title: Subcellular localization of glypican-5 is associated with dynamic motility of the human mesenchymal stem cell line U3DT

doi: 10.1371/journal.pone.0226538

Figure Lengend Snippet: (A) Electron microscopic image of negative-stained EVs. The inset shows a magnification of the boxed EV. (B) Size distribution of vesicles in EV preparations measured by image software (n = 110). (C) (F) (I) (L) GPC5-immunostained EVs (red) stained for Rab11 (green) (C), FGFR1 (green) (F), CD63 (green) (I), and ARF6 (green) (L). In (I) and (L), an Alexa Fluor 647- conjugated anti-GPC5 antibody was used to detect of GPC5-ositive particles. (D) (G) (J) (M) Line scan determination of the red bars in (C), (F), (I), and (L): GPC5 (red), others (green). (E) (H) (K) (N) Distribution of GPC5-positive particles identified by scan determinations in (D), (G), (J), and (M). n = 1,516, (E), 630 (H), 573 (K), and 835 (N). Scale bars: (A), 500 nm; (C), (F), (I), (L), 2 μm.

Article Snippet: The following antibodies and fluorescence reagents were used: anti-GPC5 antibody (MAB2607, R&D Systems, Inc.), Alexa Fluor 594 conjugated anti-GPC5 antibody (R&D Systems, Inc.), Alexa Fluor 647 conjugated anti-GPC5 antibody (R&D Systems, Inc.), anti-FGFR1 Xp rabbit monoclonal antibody (D8E4, Cell Signaling Technology), anti-Rab11A antibody (A-6: sc-166912, Santa Cruz Biotech), anti-Rab11 (D4F5)XP rabbit monoclonal antibody (Cell Signaling Technology), anti-acetyl-alpha-tubulin rabbit monoclonal antibody (D20G3, #5335, Cell Signaling Technology), Alexa Fluor 488-conjucated wheat germ agglutinin (WGA; W1126: Invitrogen), anti-CD63 monoclonal antibody (MX-49.129.5: sc-5275, Santa Cruz Biotechnology), anti-CD63 monoclonal antibody (cl 3–13: Fuji Film Co.), anti-ARF6 monoclonal antibody (3A-1: sc-7971, Santa Cruz Biotechnology), anti-ARF6 polyclonal rabbit antibody (20225-1-AP, Proteintech), Alexa Fluor 488-conjugated goat-anti-mouse IgG(H+L), F(ab’)2 fragment (#4408, Cell Signaling), Alexa Fluor 488-conjugated goat-anti-rabbit IgG(H+L), F(ab’)2 fragment (#4412, Cell Signaling Technology), Alexa Fluor 568-labeled donkey-anti-mouse IgG (H+L) (A10037, Invitrogen), Alexa Fluor 594-labeled goat anti-mouse IgG IgG(H+L), F(ab’)2 fragment (A-11020, Molecular Probes, Inc.), and Alexa Fluor 647-conjugated goat-anti-rabbit IgG(H+L), F(ab’)2 fragment (#4414, Cell Signaling Technology).

Techniques: Staining, Software

Journal: Platelets

Article Title: Protein expression in platelets from six species that differ in their open canalicular system

doi: 10.3109/09537101003611385

Figure Lengend Snippet: The antibodies used and the sequence identities of the proteins examined.

Article Snippet: Antibodies and reagents Antibodies used in this study (see ) are as follows: an anti-Arf6 rabbit polyclonal antibody produced, under contract, by Bethyl Laboratory (Montgomery, TX, USA) and described in [ 17 ], an anti-RabGDI α rabbit polyclonal antibody generated and characterized in [ 23 ]; anti-Src pp60 , anti-IQGAP1, anti-Cdc42, anti-RalA, anti-Rap1A and anti-GIT1 mouse monoclonal antibodies (BD Biosciences, San Jose, CA, USA); anti-IQGAP2 mouse monoclonal antibody (GeneTex, San Antonio, TX), USA; anti-Rac1, anti-K/H/N-Ras and anti-calmodulin (CaM) mouse monoclonal antibodies (Upstate Biotechnology, Lake Placid, NY, USA); anti- β 3 integrin polyclonal antibody (Cell Signaling, Danvers, MA, USA); anti- α -actinin mouse monoclonal antibody and anti-Arf1/3 sheep polyclonal antibody (Sigma, St Louis, MO, USA); anti-focal adhesion kinase (FAK) rabbit polyclonal antibody (EMD Chemicals Inc., Gibbstown, NJ, USA); and anti-RhoA mouse monoclonal antibody (Cytoskeleton, Denver, CO, USA).

Techniques: Sequencing

Journal: Platelets

Article Title: Protein expression in platelets from six species that differ in their open canalicular system

doi: 10.3109/09537101003611385

Figure Lengend Snippet: Protein expression in platelets from different species.

Article Snippet: Antibodies and reagents Antibodies used in this study (see ) are as follows: an anti-Arf6 rabbit polyclonal antibody produced, under contract, by Bethyl Laboratory (Montgomery, TX, USA) and described in [ 17 ], an anti-RabGDI α rabbit polyclonal antibody generated and characterized in [ 23 ]; anti-Src pp60 , anti-IQGAP1, anti-Cdc42, anti-RalA, anti-Rap1A and anti-GIT1 mouse monoclonal antibodies (BD Biosciences, San Jose, CA, USA); anti-IQGAP2 mouse monoclonal antibody (GeneTex, San Antonio, TX), USA; anti-Rac1, anti-K/H/N-Ras and anti-calmodulin (CaM) mouse monoclonal antibodies (Upstate Biotechnology, Lake Placid, NY, USA); anti- β 3 integrin polyclonal antibody (Cell Signaling, Danvers, MA, USA); anti- α -actinin mouse monoclonal antibody and anti-Arf1/3 sheep polyclonal antibody (Sigma, St Louis, MO, USA); anti-focal adhesion kinase (FAK) rabbit polyclonal antibody (EMD Chemicals Inc., Gibbstown, NJ, USA); and anti-RhoA mouse monoclonal antibody (Cytoskeleton, Denver, CO, USA).

Techniques: Expressing

Journal: Platelets

Article Title: Protein expression in platelets from six species that differ in their open canalicular system

doi: 10.3109/09537101003611385

Figure Lengend Snippet: The levels of Arf6-GTP in platelets from different species. (A) Washed platelets (2 ×108) were incubated at 37°C for 5 min, lysed, and analysed for Arf6-GTP as in Methods. An aliquot of each lysate (5%) was immunoblotted for Arf6 or RabGDIα(Total). (B) The ratios of Arf6-GTP to Arf6 total were calculated from fluorescent intensities. (C) The ratios of Arf6-GTP to RabGDIα totals were calculated and normalized to human #1.

Article Snippet: Antibodies and reagents Antibodies used in this study (see ) are as follows: an anti-Arf6 rabbit polyclonal antibody produced, under contract, by Bethyl Laboratory (Montgomery, TX, USA) and described in [ 17 ], an anti-RabGDI α rabbit polyclonal antibody generated and characterized in [ 23 ]; anti-Src pp60 , anti-IQGAP1, anti-Cdc42, anti-RalA, anti-Rap1A and anti-GIT1 mouse monoclonal antibodies (BD Biosciences, San Jose, CA, USA); anti-IQGAP2 mouse monoclonal antibody (GeneTex, San Antonio, TX), USA; anti-Rac1, anti-K/H/N-Ras and anti-calmodulin (CaM) mouse monoclonal antibodies (Upstate Biotechnology, Lake Placid, NY, USA); anti- β 3 integrin polyclonal antibody (Cell Signaling, Danvers, MA, USA); anti- α -actinin mouse monoclonal antibody and anti-Arf1/3 sheep polyclonal antibody (Sigma, St Louis, MO, USA); anti-focal adhesion kinase (FAK) rabbit polyclonal antibody (EMD Chemicals Inc., Gibbstown, NJ, USA); and anti-RhoA mouse monoclonal antibody (Cytoskeleton, Denver, CO, USA).

Techniques: Incubation